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KMID : 0604520220480020105
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Journal of the Society of Cosmetic Scientists of Korea
2022 Volume.48 No. 2 p.105 ~ p.112
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A Study on Melanin Reduction through Autophagy by 2'-Fucosyllactose
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Jung So-Young
Yoo Han-Jun
Heo Hyo-Jin
Lee So-Min
Brito Sofia
Cha Byung-Sun
Lei Lei
Lee Sang-Hun
Bin Bum-Ho
Lee Mi-Gi
Kwak Byeong-Mun
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Abstract
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2'-fucosyllactose (2'-FL) is the most abundant human milk oligosaccharide (HMO) present in breast milk, promoting the growth of beneficial microorganisms in the gut and aiding in the relief of allergic and inflammatory reactions. In this study, the anti-melanogenic effects of 2'-FL, and its potential for application in whitening cosmetics, were evaluated. MTT assay was performed on MNT-1 cells, human-derived melanocytes. 2'-FL was treated and replaced at 48 h intervals for 7 days, and it was confirmed that there was no cytotoxicity at 20 g/L or less, while a 40% reduction in melanin production was also observed. Western blot analysis of TYR and TYRP1, factors involved in melanogenesis, revealed that 2'-FL treatment reduced their expression levels. In addition, 2'-FL application and observation of the autophagy marker microtubule-associated protein 1 light chain 3 (LC3) revealed it was converted from LC3-Ι to LC3-Π, indicating increased autophagy. Likewise, confocal microscopy revealed an increase in LC3 puncta after 2'-FL treatment. Therefore, it is suggested that 2'-FL-mediated activation of autophagy reduces melanogenesis by inhibiting the expression levels of TYR and TYRP1 proteins. In conclusion, it has been confirmed that 2'-FL induces autophagy and suppresses melanin production, so its potential as a whitening cosmetic material is expected.
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KEYWORD
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2', -fucosyllactose (2', -FL), human milk oligosaccharides, autophagy, whitening, melanin
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